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Lower concentrations were used when it was not possible to make measurements at 5 mg mL 1 , either due to solubility issues (i.e

Injectable semaglutide: Injected subcutaneously into fat tissue Slow, continuous absorption from injection depot into bloodstream over 24 to 72 hours Once in bloodstream, semaglutide binds to albumin (97% protein-bound) Albumin binding creates a reservoir that slowly releases free semaglutide Terminal half-life of 7 days means each weekly injection adds to existing blood levels Steady state reached after 4 to 5 weekly injections Weekly dosing maintains plasma concentrations within therapeutic range continuously Oral semaglutide: Absorbed in stomach over 30 to 60 minutes via SNAC mechanism Rapid peak (1 hour), then distribution Same albumin binding and half-life once in bloodstream But absorption is a discrete daily event, not continuous Each missed day means zero absorption that day Daily dosing is required to maintain steady input to match continuous elimination The key difference: injectable semaglutide creates a depot that continuously feeds the bloodstream

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